Glasgow/Wetlab/Gels
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Contents |
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Gels
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Friday 10th August 2007
| Date and Reference | Description | Key | Gel |
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| 10th Aug 2007 (2) | Colony PCR after ligating phzM, phzS, DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1. | 1.phzM 2.phzM | 
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| 10th Aug 2007 (2) | Colony PCR after ligating phzM, phzS, DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2. | 1.phzS 2.phzS | 
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Monday 13th August 2007
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 13th Aug 2007 (4) | PCR of genes phzA→phzG, phzA→phzD, and phzD→phzG. Wells in the gel were made larger by putting tape on the combs in order to yield more DNA in gel extraction. | 1A.phzA→phzG 1B.phzA→phzG | 
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Friday 17th August 2007
| Date and Reference | Description | Key | Gel |
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| 17th Aug 2007 (2-4) | PstI digested minipreps of phzM and phzS in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1. | 1.-3.phzM+4/6B (PstI site mutated) 4.-6.phzM+3/20G | 
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| 17th Aug 2007 (2-4) | PstI digested minipreps of phzM and phzS in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2. | 1.-2.phzS+3/20G (1st PstI site mutated) 3.phzM+4/6B | 
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Thursday 23rd August 2007
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 23rd Aug 2007 | phzS minipreps in 4/6B and 3/20G run after the second round of site-directed mutagenesis. | 1.-5.phzS+4/6B 6.-16.phzS+3/20G | 
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| 23rd Aug 2007 | PstI digested phzS minipreps in 4/6B and 3/20G vectors after the second round of site-directed mutagenesis. | 1.-5.phzS+4/6B 6.-16.phzS+3/20G | 
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| 23rd Aug 2007 | Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 1. | 1.-7.colonies from the plate | 
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| 23rd Aug 2007 | Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 2. | 8.-12.colonies from the plate 13.neg control | 
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Friday 24th August 2007
| Date and Reference | Description | Key | Gel |
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| 24th Aug 2007 (1) | These are the miniprepped plasmids from Pr+XylR in TOPO vector. The seven colonies gave the right size product from colony PCR but failed to give right size bands after miniprepping. Wait for sequencing results. | 1.-7.colonies from Pr+XylR in TOPO transformations | 
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Monday 27th August 2007
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 27th Aug 2007 (1) | pDntA in 3/20G and 4/6B vectors digested with Roche's PvuI restriction enzyme. Results do not match up with expected fragment sizes, thus performing a PCR with "VF2" and "VR" primers. | 1.pDntA+3/20G 2.pDntA+4/6B | 
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