From IGEM07

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• Infector Detector
  • Introduction
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• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
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• Wet Lab
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Cell-Free: Our Contribution

In vitro Expression

• Attempted to make S30 E. coli cell extract and feeding solution
• Successfully used commercial S30 E. coli cell extract and feeding solution from Promega for expression of GFP
• Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
• Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution

Vesicles Formation

• Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
• Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
• Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
• Attempting to find suitable pore proteins to prolong vesicle and expression lifespan

Documentation on Registry of Parts

Documented our findings with the following Registry Additions:

Cell-Free Systems

• Homemade E. coli S30
• Commercial E. coli S30
• Commercial E. coli T7 S30
• Vesicle Encapsulation

<< Advantages of CFS | Our Contribution | Characterisation >>