From IGEM07

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

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Cell by Date: Implementation

DNA Construct Implementation
Construct Design	Concept 1	Concept 2
Stage 1: Availability	Found as BBa_I13522 in Registry	Found as BBa_E7104 in Registry.
Stage 2: Cloning / Amplification	BBa_I13522 - Lane R2 (937bp + 2079bp vector)	BBa_E7104 - Lane 11 (Missing 826bp)
Stage 3: Ligation	Not Required	Since BBa_E7104 was not functional, we made our own promoter and and ligated it to BBa_I13504 + =
Stage 4: Purification of Activator Proteins	Not Required	Not Required
Stage 5: Quality Control	Testing in vivo: Passed (Protocols and Results) Sequencing of Construct: Completed	Testing in vitro: Passed (Protocols and Results) Sequencing of Construct: Completed
Stage 6: Final Implementation Status	Ready for Testing	Ready for Testing

Chassis Construct Implementation
Chassis Design
Stage 1: Availability	Possibility of making Homemade S30 Cell Extract.	Commercially available S30 Cell Extract purchasable from Promega.
Stage 2: Preparation	Preparation: Done (Protocol)	Preparation: Done (Protocol)
Stage 3: Quality Control	Testing in vitro: Failed (Protocol and Results)	Testing in vitro: Passed (Protocol and Results)
Stage 4: Final Implementation Status	Aborted. Expression capacity of chassis was low.	Ready for Testing