Melbourne/Primary Reagents & disposables
From IGEM07
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Contents |
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Primary Reagents
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Kits
- IGEM 2007 kit
- Miniprep kit
- Gel clean up kit
- Site directed mutagenesis (stratagene)
- Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
- Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
- QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
- Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
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Cells
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Enzymes
- Melbourne/primary Restriction enzymes
- T4 Ligase
- T4 Ligase buffer 10X
- T4 Ligase buffer 2X
- DNA ladder
- phospatase
- BSA
- GoTaq
- vf2 oligonucleotide
- vR oligonucleotide
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Antibiotics
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Chemicals
- Agar
- Agarose
- Ethidium Bromide
- EDTA
- Glycerol
- Loading dye for DNA gel
- NaCl
- TRIS
- IPTG
- Xgal
- Bacto-tryptone
- Yeast Extract
- NaOH
- NZ amine
- Bacto-yeast Extract
- Ethanol
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Other
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Disposables
- Parrafilm
- Tips (10, 200, 1000 ul)
- Gloves (Large , Med, Small)
- Scalpels
- Tube racks
- Freezer boxes
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