From IGEM07

<return to list of secondary methods> <team home page>

Contents 1 Preparation of NZY media 2 Uses 3 Ingedients: 4 Method: 5 Equipment:

Preparation of NZY media

Uses

• Site-directed mutagenesis

Ingedients:

Per Liter:

• 10g NZ amine
• 5g NaCl
• 5g Bacto-yeast Extract
• NaOH to adjust pH
• Sterile water

Method:

1. Mix ingredients in Sterile water using a magnetic stirrer
2. Adjust pH to 7.5 using NaOH.

Equipment:

Magnetic Stirrer