From IGEM07

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Contents 1 Preparation of TAE Buffer 2 Uses 3 Ingedients: 4 Method: 5 Note: 6 References:

Preparation of TAE Buffer

Uses

• Buffer for gel electrophoresis

Ingedients:

Per Liter:

• 242g TRIS
• 57.1ml glacial acetic acid
• 100ml 0.5 M EDTA (pH 8.0)
• Sterile water to 1L

Method:

1. Make concentrated stock solution by mixing ingredients using a magnetic stirrer.
2. Make to 1X from concentrated stock solution by adding sterile water.

Working Solution:

• 0.04M Tris-acetate
• 0.001M EDTA

Note:

TAE has low buffering capacity and becomes exhausted during extended electrophoresis. Recirculation of buffer may be necessary. TBE buffer gives equally good resolution of DNA fragments but has significantly higher buffering capacity, making recirculation of buffer unnecessary.

References:

• T. Maniatis, E. G. Fritsch, J. Sambrook. Molecular Cloning, a Laboratory Manual.