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Contents 1 Physical data 2 Double Digest Buffers 3 Lab Location 4 Store 5 Manufacturer:

Physical data

• Description: NEB Restriction enzymes are used generally in the Lab.

NEB restriction enzymes

Enzyme	Cut site	100% buffers	Cost	No of U	Part no/data	location	In store
SpeI	5A^CTAGT3	B2	US$244	2500U @ 10,000U/ml	NEB.R0133L
HindIII	5A^AGCTT3	B2	US$53	10,000 @ 20,000U/ml	NEB.R0104S
PstI	5CTGCA^G3	B3	US$58	10,000 @ 20,000U/ml	NEB.R0140S	Lab Enz Freezer Box	YES
EcoRI	5G^AATTC3	B1,2,3&4	AU$89	10,000 @ 20,000U/ml	NEB.R0101S	Lab Enz Freezer Box	YES
HaeII	5RGCGC^Y3	B4 & B2	US$53	2,000U @ 20,000U/ml	NEB.R0107S	Igem Freezer box	YES
SalI	5G^TCGAC3	B3	US$56	2,000U @ 20,000U/ml	NEB.R0138S	Lab Enz Freezer Box	YES
XbaI	5T^CTAGA3	B2	US$63	3,000U @ 20,000U/ml	NEB.R0145S	Lab Enz Freezer Box	YES

Y= (C or T), R= (A or G) Single letter code

Double Digest Buffers

Lab Location

• Location see table
• Permision to use required? No

Store

• Available from Bio21 store? Yes
• location: [Media:melbourne Erefrigerator |E-refrigerator] Needs account card. (see Daisy).

Manufacturer:

• Manufacturer:NEB
• [listing of available enzymes]

• PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007